{"621453":{"#nid":"621453","#data":{"type":"event","title":"Glucose protects E. coli from death by the Vibrio cholerae type VI secretion","body":[{"value":"\u003Cp\u003EAbstract:\u0026nbsp;Vibrio cholerae, the causative agent of the intestinal disease cholera, interacts with other bacteria in multi-species communities in host and environmental settings. Using the harpoon-like type VI secretion system (T6SS),\u0026nbsp;V. cholerae\u0026nbsp;delivers toxic proteins into neighboring cells, causing lysis. A\u0026nbsp;V. cholerae\u0026nbsp;strain that constitutively expresses T6SS genes can effectively kill target species\u0026nbsp;Escherichia coli, Aeromonas,\u0026nbsp;and T6SS-sensistive\u0026nbsp;V. cholerae\u0026nbsp;cells. I found that addition of glucose to a standardized killing assay against\u0026nbsp;V. choleraerestores\u0026nbsp;E. coli\u0026nbsp;survival but does not restore\u0026nbsp;Aeromonas\u0026nbsp;or T6SS-sensitive\u0026nbsp;V. cholerae\u0026nbsp;survival. A growth assay revealed that\u0026nbsp;E. colidoubling time does not affect killing by\u0026nbsp;V. cholerae. Additional evidence suggests\u0026nbsp;E. coli\u0026nbsp;does not produce a diffusible molecule that represses the T6SS of\u0026nbsp;V. cholerae. Investigation by fluorescence microscopy revealed that\u0026nbsp;E. coli\u0026nbsp;cells survive in glucose even when entirely surrounded by\u0026nbsp;V. cholerae\u0026nbsp;cells, which implies that glucose causes a relevant physiological change in individual\u0026nbsp;E. coli\u0026nbsp;cells. We propose that further studies should focus on the\u0026nbsp;E. coli\u0026nbsp;capsule as a potential mechanism for surviving T6SS attacks. This study makes an unprecedented case that the outcome of microbial warfare via the T6SS can be dependent on nutrient availability.\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EHolly Nichols will be presenting at 3pm in BME 1232\u003C\/p\u003E\r\n","format":"limited_html"}],"field_summary_sentence":[{"value":"CMDI Seminar 2019"}],"uid":"35021","created_gmt":"2019-05-07 18:47:16","changed_gmt":"2019-05-07 18:47:16","author":"mavdonina3","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2019-04-29T16:00:00-04:00","event_time_end":"2019-04-29T17:00:00-04:00","event_time_end_last":"2019-04-29T17:00:00-04:00","gmt_time_start":"2019-04-29 20:00:00","gmt_time_end":"2019-04-29 21:00:00","gmt_time_end_last":"2019-04-29 21:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"620089","name":"Center for Microbial Dynamics and Infection (CMDI)"}],"categories":[],"keywords":[],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1795","name":"Seminar\/Lecture\/Colloquium"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}