{"612953":{"#nid":"612953","#data":{"type":"event","title":"PhD Defense by Yusuf Uddin","body":[{"value":"\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EIn partial fulfillment of the requirements for the degree of\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDoctor of Philosophy in Biology\u003C\/p\u003E\r\n\r\n\u003Cp\u003Ein the\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Biological Sciences\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EYusuf Uddin\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003Ewill defend his dissertation\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003E\u0026nbsp;\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EBiochemical Characterization and Cryo-EM Studies of a Highly Active Spinach Photosystem II Complex\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EThursday,\u0026nbsp;November 1,\u0026nbsp;2018\u003C\/p\u003E\r\n\r\n\u003Cp\u003E1:00\u0026nbsp;PM\u003C\/p\u003E\r\n\r\n\u003Cp\u003EIBB Room 1128\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003E\u0026nbsp;\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EThesis Advisor:\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDr. Ingeborg Schmidt-Krey\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Biological Sciences\u003C\/p\u003E\r\n\r\n\u003Cp\u003EGeorgia Institute of Technology\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003E\u0026nbsp;\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ECommittee members:\u003C\/strong\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDr.\u0026nbsp;Al Merrill\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Biological Sciences\u003C\/p\u003E\r\n\r\n\u003Cp\u003EGeorgia Institute of Technology\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDr. Bridgette Barry\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of\u0026nbsp;Chemistry and Biochemistry\u003C\/p\u003E\r\n\r\n\u003Cp\u003EGeorgia Institute of Technology\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDr.\u0026nbsp;Loren Williams\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of\u0026nbsp;Chemistry and Biochemistry\u003C\/p\u003E\r\n\r\n\u003Cp\u003EGeorgia Institute of Technology\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDr.\u0026nbsp;Nael McCarty\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDepartment of Pediatrics\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Medicine\u003C\/p\u003E\r\n\r\n\u003Cp\u003EEmory University\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003E\u0026nbsp;\u003C\/strong\u003E\u003Cstrong\u003E\u0026nbsp;\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ESummary\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EPhotosystem II (PSII) is a large, photosynthetic membrane protein complex responsible for water oxidation and the formation of oxygen on Earth. Understanding the structure of this protein is important for studying photosynthetic energy transfer and assembly mechanisms in higher plants. Recently, technological breakthroughs in the field of cryo-EM have lead to the ability to study this membrane protein by single particle analysis (SPA). This technique does not require the growth of crystals, a challenge that has hindered structural determination in higher plant PSII. In this work, the biochemical preparation of spinach PSII for cryo-EM is optimized to maintain high activity. Subunit characterization, oxygen activity, UV absorbance spectroscopy, mass spectrometry, and negative stain transmission electron microscopy were employed to characterize the spinach PSII preparation. Cryo-EM experiments resulted in a first model of a highly active C2 PSII complex, which contains all intrinsic and extrinsic subunits essential for activity.\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":"","field_summary_sentence":[{"value":"Biochemical Characterization and Cryo-EM Studies of a Highly Active Spinach Photosystem II Complex"}],"uid":"27707","created_gmt":"2018-10-18 16:15:27","changed_gmt":"2018-10-18 16:15:27","author":"Tatianna Richardson","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2018-11-01T14:00:00-04:00","event_time_end":"2018-11-01T16:00:00-04:00","event_time_end_last":"2018-11-01T16:00:00-04:00","gmt_time_start":"2018-11-01 18:00:00","gmt_time_end":"2018-11-01 20:00:00","gmt_time_end_last":"2018-11-01 20:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"100811","name":"Phd Defense"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"174045","name":"Graduate students"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}