{"610748":{"#nid":"610748","#data":{"type":"event","title":"PhD Proposal by Roxanne Glazier","body":[{"value":"\u003Cp\u003ERoxanne Glazier\u003C\/p\u003E\r\n\r\n\u003Cp\u003EBME PhD Proposal Presentation\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EFriday, September 14,\u0026nbsp;2 pm\u003C\/p\u003E\r\n\r\n\u003Cp\u003EAtwood 360, Emory University\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003ECommittee Members:\u003C\/p\u003E\r\n\r\n\u003Cp\u003EKhalid Salaita, Ph.D.\u0026nbsp;(Emory Chemistry, BME)\u003C\/p\u003E\r\n\r\n\u003Cp\u003EJennifer Curtis, Ph.D. (GT, Physics)\u003C\/p\u003E\r\n\r\n\u003Cp\u003EAndr\u0026eacute;s Garc\u0026iacute;a, Ph.D. (GT, BioE)\u003C\/p\u003E\r\n\r\n\u003Cp\u003EAdam Marcus, Ph.D. (Emory,\u0026nbsp;Cancer Biology)\u003C\/p\u003E\r\n\r\n\u003Cp\u003EPhillip Santangelo, Ph.D. (GT, BME)\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EProbing the Role of Integrin Contractile Forces in Podosome Force Balance and Function\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDuring diapedesis,\u0026nbsp;immune cells migrate through the endothelium toward infection. While scanning to invade, they extend podosomes, which are acto-adhesive structures. It is hypothesized that these podosomes test the local stiffness. This is challenging to validate because of incomplete models of podosome mechanotransduction and a lack of suitable tools. The objective of this proposal is to develop and apply DNA probes to measure integrin forces in podosomes.\u0026nbsp;The\u0026nbsp;central hypothesis\u0026nbsp;is that podosomes use integrins to achieve force balance and that these forces are important for podosome function, including stiffness sensing. Aim 1 contributes Molecular Tension Fluorescence Lifetime Imaging Microscopy (MT-FLIM), which uses FLIM-FRET to measure tension on supported bilayers. Intensity-based FRET tension probes (FTPs) will then be designed for imaging dynamics like diapedesis. The second aim applies MT-FLIM to a mimetic cell-cell podosome model to reveal the mechanisms of force balance. In Aim 3, FTPs will be combined with a diapedesis model to test whether podosomes exert tension for stiffness sensing in these junctions. The completion of these aims will provide novel methods to map receptor tension and will contribute a fundamental understanding of podosome mechanobiology.\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":"","field_summary_sentence":[{"value":"Probing the Role of Integrin Contractile Forces in Podosome Force Balance and Function"}],"uid":"27707","created_gmt":"2018-08-31 15:10:44","changed_gmt":"2018-08-31 15:10:44","author":"Tatianna Richardson","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2018-09-14T15:00:00-04:00","event_time_end":"2018-09-14T17:00:00-04:00","event_time_end_last":"2018-09-14T17:00:00-04:00","gmt_time_start":"2018-09-14 19:00:00","gmt_time_end":"2018-09-14 21:00:00","gmt_time_end_last":"2018-09-14 21:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"102851","name":"Phd proposal"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"174045","name":"Graduate students"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}