{"609218":{"#nid":"609218","#data":{"type":"event","title":"PhD Defense by Liane Tellier","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003ELiane Tellier\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EPhD Thesis Defense\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EDate:\u003C\/strong\u003E\u0026nbsp;Thursday, July 26, 2018\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ETime\u003C\/strong\u003E: 3-4 PM\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ELocation:\u003C\/strong\u003E\u0026nbsp;IBB 1128 (Suddath Room)\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ECommittee Members:\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EDr. Johnna S. Temenoff, PhD\u003C\/strong\u003E, Department of Biomedical Engineering, Georgia Tech (Advisor)\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EDr. Edward A. Botchwey, PhD\u003C\/strong\u003E, Department of Biomedical Engineering, Georgia Tech\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EDr. Robert E. Guldberg, PhD\u003C\/strong\u003E, School of Mechanical Engineering, Georgia Tech\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EDr. Claude D. Jarrett, MD\u003C\/strong\u003E, Wilmington Health Orthopedic Medical Center\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EDr. Nick J. Willett, PhD\u003C\/strong\u003E, Department of Orthopaedics, Emory University\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ETitle:\u0026nbsp;\u003C\/strong\u003EControlled heparin-based delivery strategies to promote musculoskeletal tissue regeneration after injury\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EAbstract:\u003C\/strong\u003E\u0026nbsp;Despite the prevalence of musculoskeletal disorders, one of the leading causes of work-related disability in the United States, effective therapeutic delivery to the affected musculoskeletal tissues such as bone, cartilage, and muscle remains a significant challenge.\u0026nbsp; Thus, the long term goal of this research was to develop a tunable biomaterial-based system for the delivery of regenerative therapeutics to musculoskeletal tissues.\u0026nbsp; This goal was approached through the engineering of a heparin-based microparticle (MP) system with tunable heparin sulfation, heparin content, and hydrolytic degradation, which was subsequently employed for\u0026nbsp;\u003Cem\u003Ein vitro\u003C\/em\u003E\u0026nbsp;release of an osteoinductive growth factor and\u0026nbsp;\u003Cem\u003Ein vivo\u003C\/em\u003E\u0026nbsp;delivery of a chondroprotective anti-inflammatory protein and a chemokine capable of recruiting pro-regenerative cells to muscle.\u0026nbsp;\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EIn aim 1,\u0026nbsp;hydrolytically-degradable, heparin-based MPs were fabricated containing heparin derivatives of varying levels of sulfation, and N-desulfated heparin MPs were found to efficiently load and release an osteoinductive growth factor, bone morphogenetic protein-2 (BMP-2),\u0026nbsp;\u003Cem\u003Ein vitro\u003C\/em\u003E.\u0026nbsp; In aim 2,\u0026nbsp;heparin-based MPs were loaded with tumor necrosis factor-a\u0026nbsp;stimulated gene-6 (TSG-6), an anti-inflammatory protein known to inhibit plasmin, and delivered via intra-articular injection to rat knees in the context of post-traumatic osteoarthritis.\u0026nbsp;\u0026nbsp;After 21 days, TSG-6 loaded MPs reduced cartilage damage following injury, whereas a 3X higher dose of soluble TSG-6 did not, suggesting that Hep\u003Csup\u003E-N\u003C\/sup\u003E\u0026nbsp;can enhance TSG-6 bioactivity\u0026nbsp;\u003Cem\u003Ein vivo\u003C\/em\u003E\u0026nbsp;and that Hep\u003Csup\u003E-N\u003C\/sup\u003E-based MPs can effectively deliver a chondroprotective protein for cartilage regeneration.\u0026nbsp; Finally, in aim 3,\u0026nbsp;heparin-based MPs were loaded with the chemokine stromal cell-derived factor-1a\u0026nbsp;(SDF-1a) and delivered via local injection to the supraspinatus muscle in combination with systemic delivery of the bone marrow mobilizing agent, VPC01091, following severe rotator cuff injury in rats.\u0026nbsp; Co-delivery of SDF-1a\u0026nbsp;loaded MPs and VPC01091 led to significant modulation of the inflammatory cellular milieu and mesenchymal stem cell population in muscle 3 and 7 days following injury, along with significantly more regenerating muscle fibers compared to either treatment alone.\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EOverall, in this thesis, heparin-based MPs were utilized to deliver therapeutics capable of stimulating endogenous healing processes in two unique contexts of cartilage and muscle degeneration.\u0026nbsp; Given the tunability of the MP system and the ability for heparin to bind and interact with a myriad of proteins, the application of heparin-based MPs extends beyond cartilage and muscle, and may be used for a wide range of applications where controlled release of bioactive positively-charged therapeutics is required.\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":"","field_summary_sentence":[{"value":": Controlled heparin-based delivery strategies to promote musculoskeletal tissue regeneration after injury"}],"uid":"27707","created_gmt":"2018-08-01 18:03:40","changed_gmt":"2018-08-01 18:03:40","author":"Tatianna Richardson","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2018-07-26T16:00:00-04:00","event_time_end":"2018-07-26T17:00:00-04:00","event_time_end_last":"2018-07-26T17:00:00-04:00","gmt_time_start":"2018-07-26 20:00:00","gmt_time_end":"2018-07-26 21:00:00","gmt_time_end_last":"2018-07-26 21:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"100811","name":"Phd Defense"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}