{"607467":{"#nid":"607467","#data":{"type":"event","title":"BioE PhD Defense Presentation-  Kirsten Parratt","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003EAdvisor:\u0026nbsp;\u003C\/strong\u003EKrishnendu Roy, Ph.D. (Georgia Institute of Technology, Emory University)\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ECommittee:\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003ERobert Guldberg, Ph.D.\u0026nbsp;(Georgia Institute of Technology)\u003C\/p\u003E\r\n\r\n\u003Cp\u003EHang Lu, Ph.D.\u0026nbsp;(Georgia Institute of Technology)\u003C\/p\u003E\r\n\r\n\u003Cp\u003EValeria Milam, Ph.D.\u0026nbsp;(Georgia Institute of Technology)\u003C\/p\u003E\r\n\r\n\u003Cp\u003EJohnna Temenoff, Ph.D.\u0026nbsp;(Georgia Institute of Technology, Emory University)\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003E3D Material Cytometry (3DMaC): High-throughput, high replicate Screening of Materials using flow cytometry\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EBiomaterials have become a common feature in everyday life ranging from disposable daily contact lenses to implanted devices engineered to outlast the patient. There is a great deal of unrealized commercial potential for biomaterials systems and ample interest in determining optimal biomaterials for applications such as tissue engineering and detection of biological analytes. Unlike past challenges in polymer selection, candidate biomaterials need to be tested while also accounting for the complexity of living cells and the variability in biological systems. These challenges can be partially addressed by analyzing a large number of biomaterials in a high-throughput manner with high replicate number; however, such methods are lacking.\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EThis thesis shows how flow cytometry can be adapted to the study of biomaterials. Flow cytometry allows for the automated collection of a large number of unique events in a short time period and is already widely used for cell analyses. Here, biomaterial, specifically hydrogel, constructs are fabricated and a combination of shape-, size-, and fluorescence-barcoding (SSF) enables high-throughput, high replicate, highly multiplexed analyses using imaging flow cytometry. This dissertation illustrates how this new method, 3D Material Cytometry (3DMaC), can be applied to tissue engineering and analyte detection, and discusses how the method can be extended to additional biomaterial studies.\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EBioE PhD Defense Presentation- \u0026nbsp;\u0026quot;3D MATERIAL CYTOMETRY (3DMAC): HIGH-THROUGHPUT, HIGH REPLICATE SCREENING OF MATERIALS USING FLOW CYTOMETRY\u0026quot; - Kirsten Parratt\u003C\/p\u003E\r\n","format":"limited_html"}],"field_summary_sentence":[{"value":"\u00223D MATERIAL CYTOMETRY (3DMAC): HIGH-THROUGHPUT, HIGH REPLICATE SCREENING OF MATERIALS USING FLOW CYTOMETRY\u0022"}],"uid":"27917","created_gmt":"2018-07-02 18:33:22","changed_gmt":"2018-07-02 18:33:22","author":"Laura Paige","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2018-07-20T10:00:00-04:00","event_time_end":"2018-07-20T12:00:00-04:00","event_time_end_last":"2018-07-20T12:00:00-04:00","gmt_time_start":"2018-07-20 14:00:00","gmt_time_end":"2018-07-20 16:00:00","gmt_time_end_last":"2018-07-20 16:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"65448","name":"Bioengineering Graduate Program"}],"categories":[],"keywords":[{"id":"172056","name":"go-BioE"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"10377","name":"Career\/Professional development"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003ELaura Paige\u003C\/p\u003E\r\n\r\n\u003Cp\u003E404-385-6655\u003C\/p\u003E\r\n","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}