{"603721":{"#nid":"603721","#data":{"type":"event","title":"MS Defense by Zuhui Wang","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003EZuhui Wang\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EBioE M.S. Thesis Defense\u003C\/p\u003E\r\n\r\n\u003Cp\u003ETuesday, March 27th, 2018\u003C\/p\u003E\r\n\r\n\u003Cp\u003E12:00 pm, 2110 Whitaker\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EAdvisor:\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003ERobert E. Gross, Ph.D., M.D. (Department of Neurosurgery, Emory University)\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ECommittee:\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003ELily S. Cheung, Ph.D. (ChBE, Georgia Institute of Technology)\u003C\/p\u003E\r\n\r\n\u003Cp\u003EKostas Konstantinidis, Ph.D. (CEE, Georgia Institute of Technology)\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EBioluminescence Calcium Indicator Protein and Gold Nanoparticle-enhanced Bioluminescence\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EBioluminescent proteins have been extensively used as a light emission source of many fusion proteins and have a wide range of application in bio-imaging and cell signaling studies. In our study, we focused on \u003Cem\u003EGaussia\u003C\/em\u003E luciferase (GLuc) from the marine copepod \u003Cem\u003EGaussia princeps\u003C\/em\u003E, which is the smallest known luciferase. The first part, we developed two ultrasensitive calcium sensing fusion proteins composed of a variant of GLuc, \u0026quot;slow-burn\u0026quot; Gluc (sbGLuc) and the calcium-dependent fluorescent sensor protein, GCaMP6s. Our design exhibited excellent sensitivity at physiological calcium concentration and can be explained by bioluminescence resonance energy transfer (BRET). The second part, we represented a bioluminescence enhancement strategy, which conjugates Ni-NTA (nitrilotriacetic acid) coated gold nanoparticle with 6 histidine tagged sbGLuc. One version exhibited 75% enhancement of bioluminescence compared with the non-treated sbGLuc. The new sensors together with the gold nanoparticle-enhanced bioluminescence should be useful for various studies in various fields such as neuroscience and cell biology.\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":"","field_summary_sentence":[{"value":"Bioluminescence Calcium Indicator Protein and Gold Nanoparticle-enhanced Bioluminescence"}],"uid":"27707","created_gmt":"2018-03-13 15:13:42","changed_gmt":"2018-03-13 15:13:42","author":"Tatianna Richardson","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2018-03-27T13:00:00-04:00","event_time_end":"2018-03-27T15:00:00-04:00","event_time_end_last":"2018-03-27T15:00:00-04:00","gmt_time_start":"2018-03-27 17:00:00","gmt_time_end":"2018-03-27 19:00:00","gmt_time_end_last":"2018-03-27 19:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"111531","name":"ms defense"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"174045","name":"Graduate students"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}