{"603008":{"#nid":"603008","#data":{"type":"event","title":"PhD Defense by Zunlong Ke","body":[{"value":"\u003Cp\u003EIn partial fulfillment of the requirements for the degree of\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDoctor of Philosophy in Structural Biology\u003C\/p\u003E\r\n\r\n\u003Cp\u003Ein the\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Biological Sciences\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EZunlong Ke\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003Ewill defend his dissertation\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EStructural Analysis of Virus Assembly by Cryo-Electron Tomography:\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EMeasles Virus and Respiratory Syncytial Virus\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EMonday, March 12\u003Csup\u003Eth\u003C\/sup\u003E, 2018\u003C\/p\u003E\r\n\r\n\u003Cp\u003E3:00 PM\u003C\/p\u003E\r\n\r\n\u003Cp\u003EHealth Science Research Building Auditorium (HSRB Auditorium)\u003C\/p\u003E\r\n\r\n\u003Cp\u003E1760 Haygood Dr., Atlanta, GA 30322\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003E\u0026nbsp;\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EThesis Advisor:\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDr. Elizabeth R. Wright\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDepartment of Pediatrics, School of Medicine\u003C\/p\u003E\r\n\r\n\u003Cp\u003EEmory University\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ECommittee members:\u003C\/strong\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDr. Stephen C. Harvey, Co-advisor\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Biological Sciences\u003C\/p\u003E\r\n\r\n\u003Cp\u003EGeorgia Institute of Technology\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDr. Philip J. Santangelo\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESchool of Biomedical Engineering\u003C\/p\u003E\r\n\r\n\u003Cp\u003EGeorgia Institute of Technology\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDr. Martin L. Moore\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDepartment of Pediatrics, School of Medicine\u003C\/p\u003E\r\n\r\n\u003Cp\u003EEmory University\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDr. Nael A. McCarty\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDepartment of Pediatrics, School of Medicine\u003C\/p\u003E\r\n\r\n\u003Cp\u003EEmory University\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003E\u0026nbsp;\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003E\u0026nbsp;\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EStructural Analysis of Virus Assembly by Cryo-Electron Tomography:\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EMeasles Virus and Respiratory Syncytial Virus\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003E\u0026nbsp;\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EAbstract\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EViruses, are such efficient nano-machines that they can pack all the genomic materials in a confined space essential for self-replication; and yet they are the smallest of living entities among all life forms. When studied carefully, viruses can parasitize in all living organisms. Animals, plants, bacteria, and fungi are all subject to viral infections. In this dissertation, I will be mainly focusing on investigating measles virus (MeV) and respiratory syncytial virus (RSV) by cryo-electron tomography (cryo-ET). MeV and RSV are single-stranded negative-sense RNA viruses. They are important human pathogens that can cause severe diseases; in some cases, they can lead to death.\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EMeV and RSV are known for their pleomorphic nature. Because of the inherent heterogeneity, the structural studies of these two viruses have not been well explored until recent years. Whole-cell tomography of virus-infected cells is possible and provides a much more native environment to study virus assembly as well as the authentic architecture of viral particles.\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EMeV remains a major human pathogen, with recurrent outbreaks impacting the pediatric population worldwide. To elucidate the principles governing paramyxovirus assembly and budding, we used cryo-ET to directly visualize MeV-infected human-derived cells. The three-dimensional (3D) arrangement of the MeV structural proteins including the surface glycoproteins (F and H), matrix protein (M), and the ribonucleoprotein complex (RNP) were characterized at stages of virus assembly and budding, and in released virus particles. A two-layered F-M lattice was revealed and the F-M lattice suggests that interactions between these proteins are present and may coordinate processes essential for MeV assembly. In this model, the M lattice facilitates the well-ordered incorporation and concentration of the surface glycoproteins and the RNP at sites of virus assembly.\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003ERSV is the leading cause of lower respiratory tract disease in young children, immuno-compromised adults, and the elderly. In this study, we have used cryo-ET to study the morphology and assembly of RSV from infected human cells in its close-to-native state. Our results have demonstrated that RSV is filamentous across several virus strains and cell lines by cryo-ET, cryo-immuno EM, and thin sectioning TEM techniques. Taking advantage of the whole cell tomography technique, we have resolved different stages of RSV assembly. Collectively, our results will facilitate the understanding of viral morphogenesis in RSV and other pleomorphic enveloped viruses.\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":"","field_summary_sentence":[{"value":"   Structural Analysis of Virus Assembly by Cryo-Electron Tomography:  Measles Virus and Respiratory Syncytial Virus"}],"uid":"27707","created_gmt":"2018-02-27 19:58:02","changed_gmt":"2018-02-27 19:58:02","author":"Tatianna Richardson","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2018-03-12T16:00:00-04:00","event_time_end":"2018-03-12T18:00:00-04:00","event_time_end_last":"2018-03-12T18:00:00-04:00","gmt_time_start":"2018-03-12 20:00:00","gmt_time_end":"2018-03-12 22:00:00","gmt_time_end_last":"2018-03-12 22:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"100811","name":"Phd Defense"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"174045","name":"Graduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}