{"597626":{"#nid":"597626","#data":{"type":"event","title":"PhD Proposal by Timothy Lee","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003ETimothy Lee\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EBioE PhD Proposal Presentation\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EDate:\u003C\/strong\u003E Thursday, November 2, 2017\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ETime:\u003C\/strong\u003E 2:00 PM EST\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ELocation:\u003C\/strong\u003E Engineered Biosystems Building \u0026ndash; Children\u0026rsquo;s Healthcare of Atlanta Seminar Room (EBB \u0026ndash; CHOA Seminar Rm)\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EAdvisor: \u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cem\u003ECraig R. Forest, PhD \u003C\/em\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cem\u003EGeorgia Institute of Technology, G. W. Woodruff School of Mechanical Engineering\u003C\/em\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cem\u003E\u0026nbsp;\u003C\/em\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ECommittee:\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cem\u003ER. Clay Reid, MD \u003C\/em\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cem\u003EAllen Institute for Brain Science\u003C\/em\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cem\u003E\u0026nbsp;\u003C\/em\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cem\u003EMachelle T. Pardue, PhD\u003C\/em\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cem\u003EGeorgia Institute of Technology, Coulter Department of Biomedical Engineering\u003C\/em\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cem\u003E\u0026nbsp;\u003C\/em\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cem\u003EPeter J. Yunker, PhD\u003C\/em\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cem\u003EGeorgia Institute of Technology, School of Physics\u003C\/em\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cem\u003E\u0026nbsp;\u003C\/em\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cem\u003ETodd Sulchek, PhD\u003C\/em\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cem\u003EGeorgia Institute of Technology, G. W. Woodruff School of Mechanical Engineering\u003C\/em\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EBatch processing of brain tissue sections for millimeter-scale serial section transmission electron microscopy connectomics\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EThe field of connectomics has emerged a promising approach for exploring the nature of neural circuits. A millimeter-scale connectome\u0026mdash;a neuron-to-neuron wiring diagram of a neural circuit\u0026mdash;potentially contains significant information regarding information processing and memory. The field is held back, however, by the difficulty in consistently and rapidly collecting neuroanatomical datasets with serial section transmission electron microscopy (ssTEM). In the cerebral cortex, for instance, a local circuit is contained in a cubic millimeter, but single sections\u0026mdash;obtained by cutting brain samples with a diamond knife\u0026mdash;must be \u0026ldquo;ultrathin\u0026rdquo; (\u0026lt; 40 nanometers), thus requiring 25,000 consecutive sections to be processed. Currently, the processing of ultrathin sections remains an unsolved problem that is necessary for the advancement of ssTEM connectomics. The goals of this proposal are: (1) design, model, and test a novel device that uses hydrodynamic forces and curvature-induced capillary interactions for the transport and trapping of ultrathin sections, (2) design, implement, and characterize batch processing of single sections to enable reliable processing of thousands of serial sections, and (3) design, test, and characterize automated batched section processing, enabling high-throughput and reliable section processing. In total, these aims comprise a novel platform for section processing for millimeter-scale ssTEM connectomics studies.\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":"","field_summary_sentence":[{"value":"Batch processing of brain tissue sections for millimeter-scale serial section transmission electron microscopy connectomics"}],"uid":"27707","created_gmt":"2017-10-19 17:27:31","changed_gmt":"2017-10-19 17:27:31","author":"Tatianna Richardson","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2017-11-02T15:00:00-04:00","event_time_end":"2017-11-02T17:00:00-04:00","event_time_end_last":"2017-11-02T17:00:00-04:00","gmt_time_start":"2017-11-02 19:00:00","gmt_time_end":"2017-11-02 21:00:00","gmt_time_end_last":"2017-11-02 21:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"102851","name":"Phd proposal"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"174045","name":"Graduate students"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}