{"592880":{"#nid":"592880","#data":{"type":"event","title":"PhD Defense by Candice Hovell","body":[{"value":"\u003Cp\u003EBME Defense Presentation: Candice M. Hovell\u003C\/p\u003E\r\n\r\n\u003Cp\u003EDate and Time: June 29, 2017 @ 10am EST\u003C\/p\u003E\r\n\r\n\u003Cp\u003ELocation: Marcus Nanotechnology Building, Room 1117\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EAdvisors:\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EYongTae (Tony) Kim, PhD; \u0026nbsp;Georgia Tech Mechanical Engineering\u003C\/p\u003E\r\n\r\n\u003Cp\u003ELakeshia Taite, PhD; Texas A\u0026amp;M University Department of Veterinary Medicine\u003C\/p\u003E\r\n\r\n\u003Cp\u003EGilda Barabino, PhD; Dean of Engineering \u0026nbsp;City College of New York\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003ECommittee Members:\u003C\/p\u003E\r\n\r\n\u003Cp\u003EBrandon Dixon, PhD; Georgia Tech Mechanical Engineering\u003C\/p\u003E\r\n\r\n\u003Cp\u003EEdward Botchwey, \u0026nbsp;PhD; Georgia Tech Biomedical Engineering\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003EHang\u0026nbsp;Lu, PhD; Georgia Tech\u0026nbsp;Chemical \u0026amp; Biomolecular Engineering\u003C\/p\u003E\r\n\r\n\u003Cp\u003EMaribel Vazquez, PhD; City College of New York, Biomedical Engineering\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003ETitle: Development of a Novel In Vitro Blood Brain Barrier Model for the Evaluation of Nanomedicines\u003C\/p\u003E\r\n\r\n\u003Cp\u003EIn this work, we present a novel microfluidic lumen system of the BBB (MLS-BBB) for the evaluation of multifunctional nanomedicines engineered for the treatment of medulloblastoma. \u0026nbsp;Our MLS-BBB is designed to co-culture human astrocytes (HA) and human brain vascular pericytes (HBVP) around a cylindrical lumen of human brain microvascular endothelial cells (HBMEC) within a 3D hydrogel system tuned to mimic the properties of brain extracellular matrix (ECM). Our MLS-BBB facilitates the administration of tunable shear rates to a lumen of endothelial cells in direct contact with supporting astrocyte and pericyte cells within a hydrogel system optimized to facilitate the appropriate culture of astrocytes, and is to our knowledge, the first model to do so. We employed high throughput qPCR techniques to simultaneously analyze expression of 85 BBB relevant endothelial specializations such as junctional proteins (ZO-1, Claudins, JAMs, etc.), specialized transporters (GLUT-1, CAT1, TfR, etc.) and drug resistant proteins (PgP, ABCC1, ABCC4, etc.). Our results indicate that our system provides a marked increase in physiological relevance relative to transwell culture systems. Our model was further validated through comparison to BBB spheroids, by examination of model response to perturbations of the optimized ECM composition, and examination of response to the administration of TNF\u0026alpha;, an inflammatory cytokine. Our model is currently being employed in parallel with traditional transwell systems and ex vivo brain slice cultures in the preliminary evaluation of a novel nanomedicine designed for the treatment of sonic hedgehog driven (SSH) medulloblastoma to evaluate its functional utility in such studies. The ultimate goal of organ-on-a-chip model development is eventual adaptation by the community for preclinical assessment of novel drug compounds. While our MLS-BBB could be improved in several ways, including modification to a high throughout design, by combining comprehensive characterization with an assessment of functional utility relative to standard in vitro controls, we believe our work constitutes a significant step towards the realization of this goal.\u0026nbsp;\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":"","field_summary_sentence":[{"value":"Development of a Novel In Vitro Blood Brain Barrier Model for the Evaluation of Nanomedicines"}],"uid":"27707","created_gmt":"2017-06-22 13:56:00","changed_gmt":"2017-06-22 13:56:00","author":"Tatianna Richardson","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2017-06-29T11:00:00-04:00","event_time_end":"2017-06-29T13:00:00-04:00","event_time_end_last":"2017-06-29T13:00:00-04:00","gmt_time_start":"2017-06-29 15:00:00","gmt_time_end":"2017-06-29 17:00:00","gmt_time_end_last":"2017-06-29 17:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"100811","name":"Phd Defense"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}