{"587626":{"#nid":"587626","#data":{"type":"event","title":"PhD Defense by Shereka Banton","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003EShereka Banton\u003Cbr \/\u003E\r\nBME PhD\u0026nbsp;Defense\u0026nbsp;Presentation\u003C\/strong\u003E\u003Cbr \/\u003E\r\nMarch 7th, 2017 at 9 am\u003Cbr \/\u003E\r\nManufacturing\u0026nbsp;Related Disciplines Complex (MRDC), Rm 3403\u003Cbr \/\u003E\r\n\u0026nbsp;\u003Cbr \/\u003E\r\nThesis Committee Members:\u003Cbr \/\u003E\r\nGilda Barabino, PhD (Advisor)\u003Cbr \/\u003E\r\nMichael Davis, PhD (Co-advisor)\u003Cbr \/\u003E\r\nWilbur Lam, MD-PhD\u003Cbr \/\u003E\r\nPhilip Santangelo, PhD\u003Cbr \/\u003E\r\nBetty Pace, MD\u003C\/p\u003E\r\n\r\n\u003Cp\u003EAbdullah Kutlar, MD\u003C\/p\u003E\r\n\r\n\u003Cp\u003ELakeshia Taite, PhD\u003Cbr \/\u003E\r\n\u0026nbsp;\u003Cbr \/\u003E\r\n\u003Cstrong\u003EHuman Peripheral Reticulocyte Isolation and Exosome Release\u0026nbsp;\u003Cem\u003Ein vitro\u003C\/em\u003E\u003C\/strong\u003E\u003Cbr \/\u003E\r\n\u003Cbr \/\u003E\r\nThe exosomes released by peripheral reticulocytes were originally thought to function as vehicles for protein clearance for the maturing cells.\u0026nbsp; With the emergence of exosomes as mediators of intercellular communication, a new paradigm exists for the role of reticulocyte-derived exosomes in both healthy and disease states, particularly conditions whose pathology is driven by the red blood and its precursors.\u0026nbsp; However, no standard or detailed method for the isolation of human peripheral CD71\u003Csup\u003E+\u003C\/sup\u003E\u0026nbsp;reticulocytes exists.\u0026nbsp; A combination of density-dependent and immunomagnetic approaches was\u0026nbsp;used to demonstrate a procedure to isolate human CD71\u003Csup\u003E+\u003C\/sup\u003E\u0026nbsp;reticulocytes from peripheral blood. Nearly 90% of the CD71\u003Csup\u003E+\u003C\/sup\u003E\u0026nbsp;cells were distinct from the CD71\u003Csup\u003E-\u003C\/sup\u003E\u0026nbsp;population when measured with flow cytometry detection of RNA.\u0026nbsp; CD71\u003Csup\u003E+\u003C\/sup\u003E\u0026nbsp;reticulocyte-derived exosomes were then isolated and\u0026nbsp;analyzed after incubation\u0026nbsp;\u003Cem\u003Ein vitro\u003C\/em\u003E,\u0026nbsp;the first such demonstration of these phenomena using these cells.\u0026nbsp; These findings form the basis for more targeted and mechanistic studies into the role of reticulocyte-derived exosomes in pathologies like sickle cell disease.\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":"","field_summary_sentence":[{"value":"Human Peripheral Reticulocyte Isolation and Exosome Release in vitro"}],"uid":"27707","created_gmt":"2017-02-20 15:40:29","changed_gmt":"2017-02-20 15:40:29","author":"Tatianna Richardson","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2017-03-07T09:00:00-05:00","event_time_end":"2017-03-07T11:00:00-05:00","event_time_end_last":"2017-03-07T11:00:00-05:00","gmt_time_start":"2017-03-07 14:00:00","gmt_time_end":"2017-03-07 16:00:00","gmt_time_end_last":"2017-03-07 16:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"100811","name":"Phd Defense"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78771","name":"Public"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}