{"56218":{"#nid":"56218","#data":{"type":"event","title":"ChBE Spring2007 Seminar Series","body":[{"value":"\u003Cp\u003ESusan Daniel, a Post-doc in the Department of Chemistry at Texas A \u0026amp; M University, presents \u003Cem\u003EProtein Mobility, Filtering, and Separation in Model Cell Membranes\u003C\/em\u003E as part of ChBE\u0027s spring seminar series.\n\u003C\/p\u003E\n\u003Cp\u003E* Refreshments will be served at 3:30 PM in the Lower Level 1 Gossage Atrium\u003Cbr \/\u003E\n* Lecture commences at 4:00 PM in L1255 in the Ford ES\u0026amp;T Building\n\u003C\/p\u003E\n\u003Cp\u003ESeminar Abstract\u003Cbr \/\u003E\nInvestigating how biomolecules behave in cell membranes gives us insight that can be used to\u003Cbr \/\u003E\ncreate better assays, sensors, and devices that mimic the cell surface. Applications for these\u003Cbr \/\u003E\ndevices include rapid combinatorial analysis of drug targets, biosensors for toxin detection, and\u003Cbr \/\u003E\nproteomics research. Solid-supported lipid bilayers (SLBs) are an excellent platform for\u003Cbr \/\u003E\nmimicking the surface chemistry of cells. However, there are several drawbacks to these\u003Cbr \/\u003E\nplatforms. First, proteins can lose their mobility in these systems, impairing their function.\u003Cbr \/\u003E\nSecond, there is no good way to discriminate between analytes that bind to the same surface\u003Cbr \/\u003E\nligand within these platforms. Third, separation, purification, and formation of arrays of\u003Cbr \/\u003E\nmembrane species is difficult, impeding the progress of rapid combinatorial assaying of\u003Cbr \/\u003E\nmembrane proteins.\n\u003C\/p\u003E\n\u003Cp\u003EResults will be presented on studies conducted to understand these issues and strategies to\u003Cbr \/\u003E\novercome them. By investigating the behavior of protein-protein interactions on SLBs, we found\u003Cbr \/\u003E\nthat protein-packing influences the point at which diffusion is arrested in these systems. To\u003Cbr \/\u003E\nimprove binding specificity, we devised a system for size-selective discrimination of protein\u003Cbr \/\u003E\nanalytes that bind to the same ligand, by incorporating poly(ethylene glycol) (PEG) lipopolymers\u003Cbr \/\u003E\ninto SLBs. Using our platform, we were able to achieve discrimination of several orders of\u003Cbr \/\u003E\nmagnitude. Finally, we developed a technique to separate membrane species within an SLB:\u003Cbr \/\u003E\nbilayer chromatography. Results will be presented that show our separation method is sensitive\u003Cbr \/\u003E\nenough to differentiate isomers of dye-labeled lipids and is currently being extended to the\u003Cbr \/\u003E\nseparation of membrane proteins.\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"Susan Daniel presents \u003Cem\u003EProtein Mobility, Filtering, and Separation in Model Cell Membranes\u003C\/em\u003E as part of ChBE\u0027s spring seminar series.","format":"limited_html"}],"field_summary_sentence":[{"value":"Susan Daniel presents seminar"}],"uid":"27255","created_gmt":"2010-05-20 11:47:16","changed_gmt":"2016-10-08 01:51:37","author":"Josie Giles","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2007-01-31T15:00:00-05:00","event_time_end":"2007-01-31T16:00:00-05:00","event_time_end_last":"2007-01-31T16:00:00-05:00","gmt_time_start":"2007-01-31 20:00:00","gmt_time_end":"2007-01-31 21:00:00","gmt_time_end_last":"2007-01-31 21:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"1240","name":"School of Chemical and Biomolecular Engineering"}],"categories":[],"keywords":[{"id":"1303","name":"chbe"},{"id":"1704","name":"chemical \u0026 biomolecular engineering"},{"id":"2437","name":"lecture"},{"id":"166896","name":"seminar"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1795","name":"Seminar\/Lecture\/Colloquium"}],"invited_audience":[],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cstrong\u003EJosie Giles\u003C\/strong\u003E\u003Cbr \/\u003ESchool of Chemical \u0026amp; Biomolecular Engineering\u003Cbr \/\u003E\u003Ca href=\u0022http:\/\/www.gatech.edu\/contact\/index.html?id=jg234\u0022\u003EContact Josie Giles\u003C\/a\u003E\u003Cbr \/\u003E\u003Cstrong\u003E404-385-2299\u003C\/strong\u003E","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}