Jeff Jianzhong Xi,      PhD,        Department of Biomedical Engineering,   Peking University

May Dongmei Wang,   PhD,     Department of Biomedical Engineering,   Georgia Institute of Technology & Emory University 

Committee Members:

Huaiqiu Zhu,    PhD,    Department of Biomedical Engineering,    Peking University

Haifeng Chen,   PhD,         Department of Biomedical Engineering,   Peking University

Yangming Wang,   PhD,        Institute of Molecular Medicine,    Peking University

Title: Construction of Cas9-Avd system to Enhance Homologous Recombination Efficiency

Abstract: Homologous recombination is responsible for the maintenance of genome integrity. By introducing an artificial DNA template as donor, certain exogenous sequences could be inserted into genome precisely. But the efficiency is low either in mammlian cell lines or embryos. In my proposal, a novel strategy to enhance homologous recombination efficiency is described and constructed, based on Avidin and Biotin interaction mechanism. This system works well based on primary data and shows huge potential to application.