{"477421":{"#nid":"477421","#data":{"type":"event","title":"PhD Proposal by Hao Zhang","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003EHao Zhang\u003C\/strong\u003E\u003C\/p\u003E\u003Cp\u003EPhD\u0026nbsp;Proposal Presentation\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003EDate\u003C\/strong\u003E: December 17, 2015 (Wednesday)\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003ETime\u003C\/strong\u003E: 9:00 pm (Beijing) \/ 8:00 am (Atlanta)\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003ELocation\u003C\/strong\u003E:\u0026nbsp;\u003C\/p\u003E\u003Cp\u003ENew 1st COE Building,Room 208 (PKU campus at Beijing)\u0026nbsp;\u003C\/p\u003E\u003Cp\u003EU.A. Whitaker Building, Mclnitre Conf. Room (GT campus at Atlanta)\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003EAdvisor:\u003C\/strong\u003E\u003C\/p\u003E\u003Cp\u003EPeng Xi, \u0026nbsp; \u0026nbsp; \u0026nbsp;PhD, \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;Department of Biomedical Engineering, \u0026nbsp; Peking University\u003C\/p\u003E\u003Cp\u003ECheng Zhu, \u0026nbsp; PhD, \u0026nbsp; \u0026nbsp; Department of Biomedical Engineering, \u0026nbsp; Georgia Institute of Technology \u0026amp; Emory University\u0026nbsp;\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003ECommittee Members:\u003C\/strong\u003E\u003C\/p\u003E\u003Cp\u003ETianyu Xie, \u0026nbsp; \u0026nbsp;PhD, \u0026nbsp; \u0026nbsp;Department of Biomedical Engineering, \u0026nbsp; \u0026nbsp;Peking University\u003C\/p\u003E\u003Cp\u003EChanghui Li, \u0026nbsp; PhD, \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp; Department of Biomedical Engineering, \u0026nbsp; Peking University\u003C\/p\u003E\u003Cp\u003EJuntao Gao, \u0026nbsp; PhD, \u0026nbsp; \u0026nbsp; \u0026nbsp; \u0026nbsp;TNLIST, \u0026nbsp; \u0026nbsp;Tsinghua University\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003ETitle:\u0026nbsp;\u003C\/strong\u003ESingle Molecular Orientation Detection Microscopy\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003EAbstract: I\u003C\/strong\u003En the past, fluorescence polarization techniques with wide-field, confocal, two-photon excitation (TPE), total internal reflection fluorescence (TIRF)\u0026nbsp;and fluorescence recovery after photobleaching (FRAP)\u0026nbsp;techniques were used to study protein structure and function. All these techniques were obstructed by the same problem: the observation volume is usually a complex system with more than one emitter. To reduce the number of molecules within an observation volume is crucial, as well as to find parameters describing the system instead of a single orientation. Utilizing the tunable transition rates of ON\/OFF states of organic dyes or PA-FPs, single molecular orientation detection (SMOD) microsocpy introduces single molecular localization technique into fluorescence polarization imaging.\u0026nbsp;Through imaging both intensity and dipole orientation of fluorescence probes in complicated systems at single molecular accuracy, Single Molecular Orientation Detection (SMOD) microscopy would be a powerful technique in studying structural organization of proteins and lipids.\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003E \u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":"","field_summary_sentence":[{"value":"Single Molecular Orientation Detection Microscopy"}],"uid":"27707","created_gmt":"2015-12-09 11:05:04","changed_gmt":"2016-10-08 02:15:10","author":"Tatianna Richardson","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2015-12-17T12:00:00-05:00","event_time_end":"2015-12-17T14:00:00-05:00","event_time_end_last":"2015-12-17T14:00:00-05:00","gmt_time_start":"2015-12-17 17:00:00","gmt_time_end":"2015-12-17 19:00:00","gmt_time_end_last":"2015-12-17 19:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"102851","name":"Phd proposal"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78771","name":"Public"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}