{"394991":{"#nid":"394991","#data":{"type":"event","title":"PhD Defense by J.T. Shoemaker","body":[{"value":"J.T. Shoemaker\u003Cbr \/\u003EPhD Defense\u003Cbr \/\u003EDate: Monday, April 20th, 2015\u003Cbr \/\u003ETime: 2PM\u003Cbr \/\u003ELocation: Marcus Nanotechnology 1117-1118\u003Cbr \/\u003E\u003Cbr \/\u003E\u003Cstrong\u003ECOMMITTEE MEMBERS:\u003Cbr \/\u003E\u003C\/strong\u003EMichelle LaPlaca, Ph.D. (Advisor)\u003Cbr \/\u003ERavi Bellamkonda, Ph. D.\u003Cbr \/\u003EEdward Botchwey, Ph.D.\u003Cbr \/\u003EMal\u00fa Tansey, Ph. D. (Emory University)\u003Cbr \/\u003EShean Phelps, M.D., MPH, FAAFP (GTRI)\u003Cbr \/\u003E\u003Cbr \/\u003E\u003Cstrong\u003ETITLE: \u003C\/strong\u003E\u003Cbr \/\u003EDevelopment of an \u003Cem\u003Ein vitro\u003C\/em\u003E model of neuroinflammation for studying secondary injury mechanisms in traumatic brain injury\u003Cbr \/\u003E\u003Cstrong\u003E\u003Cbr \/\u003E\u003C\/strong\u003E\u003Cstrong\u003EABSTRACT:\u003Cbr \/\u003E\u003C\/strong\u003ETraumatic brain injury (TBI) is a major public health concern due to its incidence (1.7 million cases in the U.S. annually) and lack of treatment options. Despite the development of promising therapies using animal models, none have translated successfully into clinical trials. The difficulty stems from the complexity of the response to an injury with causes that are highly variable. Numerous biochemical processes are triggered in response to TBI. The signaling cascades associated with inflammation are integral components of the secondary injury mechanisms that result in long-term deficits post-injury. Many of these cascades are initiated through microglial activation, which can be induced through post-injury death of, or cytokine release by other cell types. These signals are propagated by the same mechanisms as well as by signaling from other microglia. Although \u003Cem\u003Ein vivo\u003C\/em\u003E models have the inherent advantage of using an intact animal in which the all of the systems involved in the injury response are present, they lack the controllability of cell culture models. \u003Cem\u003EIn vitro\u003C\/em\u003E tissue models provide a highly tunable environment in which \u003Cem\u003Ein vivo\u003C\/em\u003E-like phenomena can be simulated in a controlled, reproducible fashion. Further, three-dimensional (3-D) cell culture systems provide an environment in which cellular function is closer to \u003Cem\u003Ein vivo\u003C\/em\u003E than is observed using traditional two-dimensional culture methods. Such models are ideally suited for studying and deciphering a complex tissue response such as that observed in TBI. The objective of this research was to generate a 3-D culture system that models the secondary injury mechanisms of TBI \u003Cem\u003Ein vitro\u003C\/em\u003E. This goal was addressed by: 1) creating a novel 3-D multitypic neural cell culture system composed of primary neurons, astrocytes, and microglia derived from pre- and post-natal rat central neural tissue, 2) confirming the potential for inflammation in the system through chemical stimulation, and 3) devising a method of mechanical injury to elicit the inflammation response in a manner consistent with what is observed in TBI. Overall, the research presented here sheds light on the\u003Cem\u003E in vitro\u003C\/em\u003E microglial response to insult using a novel culture method, the utility of which extends beyond the purpose for which it was designed.","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":"","field_summary_sentence":[{"value":"Development of an in vitro model of neuroinflammation for studying secondary injury mechanisms in traumatic brain injury"}],"uid":"27707","created_gmt":"2015-04-09 12:40:12","changed_gmt":"2016-10-08 02:11:35","author":"Tatianna Richardson","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2015-04-20T15:00:00-04:00","event_time_end":"2015-04-20T17:00:00-04:00","event_time_end_last":"2015-04-20T17:00:00-04:00","gmt_time_start":"2015-04-20 19:00:00","gmt_time_end":"2015-04-20 21:00:00","gmt_time_end_last":"2015-04-20 21:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"1612","name":"BME"},{"id":"1366","name":"defense"},{"id":"1808","name":"graduate students"},{"id":"913","name":"PhD"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78771","name":"Public"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}