{"325611":{"#nid":"325611","#data":{"type":"event","title":"Emergent Behaviors of Integrated Cellular Systems Distinguished Lecture","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003E\u0022iPS Cell Technology, Gene Editing \u0026amp; Disease Research\u0022\u003Cbr \/\u003E\u003Cbr \/\u003ERudolf Jaenisch, MD\u003Cbr \/\u003EProfessor of Biology\u003Cbr \/\u003EMassachusetts Institute of Technology\u003C\/strong\u003E\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003EWhitehead Institute - Founding Member\u003Cbr \/\u003E\u003Cbr \/\u003E\u003C\/strong\u003E\u003C\/p\u003E\u003Cp\u003EThe recent demonstration of in vitro reprogramming using transduction of four transcription factors by Yamanaka and colleagues represents a major advance in the field. However, major questions regarding the mechanism of in vitro reprogramming need to be understood and will be one focus of the talk. A major impediment in realizing the potential of ES and iPS cells to study human diseases is the inefficiency of gene targeting. Methods based on Zn finger or TALEN mediated genome editing have allowed us to overcome the inefficiency of homologous recombination in human pluripotent cells. Using these genome editing approaches we have established efficient protocols to target expressed and silent genes in human ES and iPS cells. The most recent advance comes from the use of the CRISPR\/ Cas9 system to engineer ES cells and mice. This technology allows the simultaneous editing of multiple genes and will facilitate establishing relevant models to study human disease.\u003Cbr \/\u003E\u003Cbr \/\u003E We have used this technology to generate isogenic pairs of cells that differ exclusively at a disease causing mutation. The talk will describe the use of isogenic pairs of mutant and control iPS cells to establish in vitro systems for the study of diseases such as Parkinson\u2019s and Rett syndrome.\u003C\/p\u003E\u003Cp\u003E\u003Cstrong\u003E\u003Cbr \/\u003E\u003C\/strong\u003E\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EEmergent Behaviors of Integrated Cellular Systems Distinguished Lecture -\u0026nbsp;\u0022iPS Cell Technology, Gene Editing \u0026amp; Disease Research\u0022 - Rudolf Jaenisch, MD - Massachusetts Institute of Technology\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":[{"value":"\u0022iPS Cell Technology, Gene Editing \u0026 Disease Research\u0022 - Rudolf Jaenisch, MD - Massachusetts Institute of Technology"}],"uid":"27195","created_gmt":"2014-09-17 09:10:42","changed_gmt":"2017-04-13 21:21:41","author":"Colly Mitchell","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2014-09-18T17:00:00-04:00","event_time_end":"2014-09-18T18:00:00-04:00","event_time_end_last":"2014-09-18T18:00:00-04:00","gmt_time_start":"2014-09-18 21:00:00","gmt_time_end":"2014-09-18 22:00:00","gmt_time_end_last":"2014-09-18 22:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"related_links":[{"url":"http:\/\/ebics.net\/","title":"EBICS website"},{"url":"http:\/\/wi.mit.edu\/news\/tag\/lab\/jaenisch-lab","title":"Jaenisch lab"}],"groups":[{"id":"1292","name":"Parker H. Petit Institute for Bioengineering and Bioscience (IBB)"}],"categories":[],"keywords":[],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1795","name":"Seminar\/Lecture\/Colloquium"}],"invited_audience":[{"id":"78751","name":"Undergraduate students"},{"id":"78761","name":"Faculty\/Staff"},{"id":"174045","name":"Graduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003E\u003Ca href=\u0022mailto:lakeita.servance@ibb.gatech.edu\u0022\u003ELakeita Servance\u003C\/a\u003E\u003C\/p\u003E","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}