{"290111":{"#nid":"290111","#data":{"type":"event","title":"Bioengineering Seminar Series","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003E\u201cInterrogating Cell-to-Cell Heterogeneity by Stochastic Profiling\u201d\u003Cbr \/\u003E\u003Cbr \/\u003EKevin Janes, PhD\u003C\/strong\u003E\u003Cbr \/\u003E\u003Cstrong\u003EAssistant Professor\u003C\/strong\u003E\u003Cbr \/\u003E\u003Cstrong\u003EDepartment of Biomedical Engineering\u003C\/strong\u003E\u003Cbr \/\u003E\u003Cstrong\u003EUniversity of Virginia\u003Cbr \/\u003E\u003Cbr \/\u003E\u003C\/strong\u003ERegulated changes in gene expression underlie many biological processes, but globally profiling cell-to-cell variations in transcriptional regulation is problematic when measuring single cells.\u0026nbsp; The Janes lab has developed an approach, called stochastic profiling, that applies probability theory to transcriptome-wide measurements of small pools of cells to identify single-cell regulatory heterogeneities (Nat Methods 7:311-7 [2010]).\u0026nbsp; In the first half of the talk, Janes will discuss a two-state regulatory circuit that was identified by stochastic profiling (Nat Cell Biol 16:345-56 [2014]).\u0026nbsp; The circuit involves TGFb-family signaling and the junD transcription factor, which are asynchronously activated in 3D breast epithelial cultures to coordinate normal morphogenesis.\u0026nbsp; The circuit also appears to be re-initiated during the early stages of basal-like breast cancer, contributing to the mosaicked expression patterns observed clinically by histology.\u0026nbsp; In the second half of the talk, Janes will talk about work in progress that applies stochastic profiling as a tool for uncovering the mechanistic basis of phenotypes that are incompletely penetrant.\u0026nbsp; Regulatory-state frequencies are matched to downstream phenotype frequencies to converge upon a tractable set of candidate states worthy of follow-up experimentation.\u0026nbsp; Using the ErbB2 oncoprotein as a model trigger for an incompletely penetrant phenotype, they identify a handful of surprising candidates that significantly affect penetrance when perturbed.\u0026nbsp; Stochastic profiling remains the only method compatible with cells microdissected in situ and thereby opens exciting opportunities in the areas of tissue morphogenesis and cancer\u003Cstrong\u003E.\u003C\/strong\u003E\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EThe Bioengineering Seminar Series is a joint seminar series between the Petit Institute and the Biomedical Engineering department. Seminars are held on Tuesdays or Thursdays between 11am-12pm in Petit Institute, room 1128, unless otherwise indicated.\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":[{"value":"\u201cInterrogating Cell-to-Cell Heterogeneity by Stochastic Profiling\u201d - Kevin Janes, PhD - University of Virginia"}],"uid":"27959","created_gmt":"2014-04-11 13:38:42","changed_gmt":"2017-04-13 21:22:42","author":"Karen Ethier","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2014-09-23T16:00:00-04:00","event_time_end":"2014-09-23T17:00:00-04:00","event_time_end_last":"2014-09-23T17:00:00-04:00","gmt_time_start":"2014-09-23 20:00:00","gmt_time_end":"2014-09-23 21:00:00","gmt_time_end_last":"2014-09-23 21:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"related_links":[{"url":"http:\/\/bme.virginia.edu\/janes\/index.html","title":"Janes lab website"}],"groups":[{"id":"1292","name":"Parker H. Petit Institute for Bioengineering and Bioscience (IBB)"},{"id":"65448","name":"Bioengineering Graduate Program"}],"categories":[],"keywords":[{"id":"11877","name":"BioE Seminar"},{"id":"248","name":"IBB"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1795","name":"Seminar\/Lecture\/Colloquium"}],"invited_audience":[{"id":"78751","name":"Undergraduate students"},{"id":"78761","name":"Faculty\/Staff"},{"id":"174045","name":"Graduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003EFaculty host: \u003Ca href=\u0022%20melissa.kemp@bme.gatech.edu\u0022\u003EMelissa Kemp, PhD\u003C\/a\u003E\u003C\/p\u003E","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}