{"173961":{"#nid":"173961","#data":{"type":"event","title":"Prof. Konstantin Lukyanov, Inst. of Bioorganic Chemistry, Moscow RUSSIA","body":[{"value":"\u003Cp\u003EProf. Konstantin Lukyanov, Inst. of Bioorganic Chemistry, Moscow RUSSIA\u003C\/p\u003E\u003Cp\u003E\u003Cem\u003E\u003Cstrong\u003EUnusual fluorescent proteins: design and applications\u003C\/strong\u003E\u003C\/em\u003E\u003C\/p\u003E\u003Cp\u003EThe study and applications of fluorescent proteins has gone a long way from a subject of highly specialized research to a widely known and indispensable tool for \u003Cem\u003Ein vivo\u003C\/em\u003E labeling, and from a single known member\u00beGreen Fluorescent Protein from \u003Cem\u003EAequorea victoria\u003C\/em\u003E\u00beto hundreds of natural proteins of different colors with still poorly studied phylogeny and biological significance [1].\u003C\/p\u003E\u003Cp\u003EMy talk will be focused on fluorescent proteins with \u201cunusual\u201d spectral or biochemical characteristics. In particular, we developed KillerRed \u2013 phototoxic fluorescent protein capable of light-induced production of reactive oxygen species (ROS) [2]. KillerRed allows light-induced ROS production to be strictly localized to different compartments and subcompartments within a cell. It was shown that KillerRed-mediated oxidative stress at different locations results in clearly different molecular events and cell responses (e.g., triggering apoptosis for mitochondria-localized KillerRed, or DNA damage and temporal blockage of cell division for chromatin-localized KillerRed) [2, 3].\u003C\/p\u003E\u003Cp\u003ERecently, we designed CFP variant with an anionic tryptophan-based chromophore [4]. In contrast to green and red fluorescent proteins usually containing anionic tyrosine-based chromophores, no charged states of the CFP chromophore have been described before. We believe that switching between protonated and deprotonated tryptophan in chromophores of fluorescent proteins represents a new unexplored strategy to control their spectral properties.\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Col\u003E\u003Cli\u003EChudakov D.M., Matz M.V., Lukyanov S., Lukyanov K.A\u003Cem\u003E.\u003C\/em\u003E Fluorescent proteins and their applications in imaging living cells and tissues. Physiol. Rev. 2010, 90, 1103-1163.\u003C\/li\u003E\u003Cli\u003EBulina M.E., Chudakov D.M., Britanova O.V., Yanushevich Y.G., Staroverov D.B., Chepurnykh T.V., Merzlyak E.M., Shkrob M.A., Lukyanov S., Lukyanov K.A. A genetically encoded photosensitizer. Nature Biotechnol. 2006, 24, 95-99.\u003C\/li\u003E\u003Cli\u003ESerebrovskaya EO, Gorodnicheva TV, Ermakova GV, Solovieva EA, Sharonov GV, Zagaynova EV, Chudakov DM, Lukyanov S, Zaraisky AG, Lukyanov KA\u003Cem\u003E.\u003C\/em\u003E Light-induced blockage of cell division with a chromatin-targeted phototoxic fluorescent protein. Biochem. J. 2011, 435, 65-71.\u003C\/li\u003E\u003C\/ol\u003E\u003Col start=\u00224\u0022\u003E\u003Cli\u003ESarkisyan KS, Yampolsky IV, Solntsev KM, Lukyanov SA, Lukyanov KA, Mishin AS. Tryptophan-based chromophore in fluorescent proteins can be anionic. Sci Rep. 2012, 2, 608.\u003C\/li\u003E\u003C\/ol\u003E\u003Cp\u003EFor more information contact \u003Ca href=\u0022mailto:kyril.solntsev@chemistry.gatech.edu\u0022\u003EDr. Kyril Solntsev\u003C\/a\u003E (404-385-7131).\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EProf. Konstantin Lukyanov, Inst. of Bioorganic Chemistry, Moscow RUSSIA\u003C\/p\u003E\u003Cp\u003E\u003Cem\u003E\u003Cstrong\u003EUnusual fluorescent proteins: design and applications\u003C\/strong\u003E\u003C\/em\u003E\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":"","uid":"27275","created_gmt":"2012-11-29 09:15:31","changed_gmt":"2016-10-08 02:01:26","author":"Shirley Tomes","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2012-12-11T15:00:00-05:00","event_time_end":"2012-12-11T16:00:00-05:00","event_time_end_last":"2012-12-11T16:00:00-05:00","gmt_time_start":"2012-12-11 20:00:00","gmt_time_end":"2012-12-11 21:00:00","gmt_time_end_last":"2012-12-11 21:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"hg_media":{"173971":{"id":"173971","type":"image","title":"Lukyanov photo","body":null,"created":"1449179012","gmt_created":"2015-12-03 21:43:32","changed":"1475894816","gmt_changed":"2016-10-08 02:46:56","alt":"Lukyanov photo","file":{"fid":"195800","name":"lukyanov_photo.jpg","image_path":"\/sites\/default\/files\/images\/lukyanov_photo_0.jpg","image_full_path":"http:\/\/hg.gatech.edu\/\/sites\/default\/files\/images\/lukyanov_photo_0.jpg","mime":"image\/jpeg","size":86166,"path_740":"http:\/\/hg.gatech.edu\/sites\/default\/files\/styles\/740xx_scale\/public\/images\/lukyanov_photo_0.jpg?itok=3ORh8QMC"}}},"media_ids":["173971"],"groups":[{"id":"85951","name":"School of Chemistry and Biochemistry"}],"categories":[],"keywords":[],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1795","name":"Seminar\/Lecture\/Colloquium"}],"invited_audience":[],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003EShirley Tomes (404-894-0591) \u003Ca href=\u0022mailto:shirley.tomes@chemistry.gatech.edu\u0022\u003Eshirley.tomes@chemistry.gatech.edu\u003C\/a\u003E\u003C\/p\u003E","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}