{"125051":{"#nid":"125051","#data":{"type":"event","title":"Prof. Philip Dawson, The Scripps Research Institute","body":[{"value":"\u003Cp\u003EProf. Philip Dawson, The Scripps Research Institute\u003C\/p\u003E\u003Cp\u003E\u003Cem\u003E\u003Cstrong\u003EChemoselective strategies for the synthesis of proteins and labeling of nanoparticles\u003C\/strong\u003E\u003C\/em\u003E\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\u003Cp\u003EChemical ligation approaches have become essential tools for the engineering of complex molecules including proteins, nucleic acids and nanoparticles. What makes these reactions so useful is their compatibility with the biological \u0022solvent\u0022 water, and a high level of chemoselectivity that enables their application in complex molecular environments. We have worked to develop several ligation chemistries that are highly chemoselective and have sufficient ligation rates to be useful at low concentrations. In one case, the use of hydrolysis resistant thioester peptides that undergo inter\u2010 and intramolecular acyl transfer enables the total synthesis of proteins. The optimization of the ligation methodology, improved routes to the required peptide intermediates, and application of these methods to complex targets will be presented. Another challenge is the covalent assembly of macromolecules and nanoparticles. In these systems, a \u0022native\u0022 linkage is irrelevant and the main criteria for a successful ligation methodology are fast reaction rates and high chemoselectivity. We have found that aniline catalyzed hydrazone and oxime reactions enable the controlled assembly and disassembly of macromolecular complexes in aqueous solution at micromolar concentrations. The scope of these reactions and new approaches for their catalysis will be discussed.\u003C\/p\u003E\u003Cp\u003EFor more information contact \u003Ca href=\u0022http:\/\/mailto:lyon@chemistry.gatech.edu\u0022\u003EProf. Andrew Lyon\u003C\/a\u003E (404-894-4090).\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EProf. Philip Dawson, The Scripps Research Institute\u003C\/p\u003E\u003Cp\u003E\u003Cem\u003E\u003Cstrong\u003EChemoselective strategies for the synthesis of proteins and labeling of nanoparticles\u003C\/strong\u003E\u003C\/em\u003E\u003C\/p\u003E\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":"","uid":"27275","created_gmt":"2012-04-19 13:00:10","changed_gmt":"2016-10-08 01:58:45","author":"Shirley Tomes","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2012-04-20T21:00:00-04:00","event_time_end":"2012-04-20T22:00:00-04:00","event_time_end_last":"2012-04-20T22:00:00-04:00","gmt_time_start":"2012-04-21 01:00:00","gmt_time_end":"2012-04-21 02:00:00","gmt_time_end_last":"2012-04-21 02:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"related_links":[{"url":"http:\/\/www.scripps.edu\/research\/faculty\/dawson","title":"Prof. Philip Dawson, Scripps"}],"groups":[{"id":"85951","name":"School of Chemistry and Biochemistry"}],"categories":[],"keywords":[],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1795","name":"Seminar\/Lecture\/Colloquium"}],"invited_audience":[],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cp\u003EShirley Tomes (404-894-0591) \u003Ca href=\u0022mailto:shirley.tomes@chemistry.gatech.edu\u0022\u003Eshirley.tomes@chemistry.gatech.edu\u003C\/a\u003E\u003C\/p\u003E","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}