event

(09-1030) Prof. Samuel E. Butcher, University of Wisconsin-Madison

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Prof. Samuel E. Butcher, University of Wisconsin-Madison

RNA-protein interactions that drive spliceosome assembly

The spliceosome is a massive 2 MDa assembly of 5 RNAs (U1, U2, U4, U5 and U6)and more than 100 proteins. This ribonucleoprotein complex is responsible for precursor messenger RNA (pre‐mRNA) splicing, an essential step in eukaryotic gene expression. Biochemical and genetic data indicate that the spliceosomal U2 and U6 RNAs are involved in active site formation, and directly contact the pre‐mRNA substrate during splicing catalysis. Prior to catalysis, the spliceosomal RNAs undergo large‐scale conformational rearrangements during assembly. These rearrangements are protein catalyzed, and the structure of protein‐RNA interactions involved in this process will be presented. Additionally, recent data that shed light on the mechanism of the protein‐mediated RNA conformational rearrangements during spliceosome assembly will be discussed.

For more information contact Prof. Nick Hud (404-385-1162) or Prof. Bridgette Barry (404-385-6985).

Status

  • Workflow Status:Published
  • Created By:Shirley Tomes
  • Created:10/22/2009
  • Modified By:Fletcher Moore
  • Modified:10/07/2016

Keywords