"Pseudomonas aeruginosa is a Gram-negative pathogen that regulates coordinated cooperative behaviors using quorum sensing (QS), a form of cell-to-cell signaling. QS in P. aeruginosa is tightly controlled by three intertwined, hierarchical systems, which can be re-regulated in some clinical strains. In P. aeruginosa, QS has predominantly been studied in well mixed liquid cultures, but in order to better understand QS dynamics during infection or natural environments, there is a need to study QS in more structured environments. Previous work has identified that there is QS heterogeneity between cells in well mixed planktonic cultures, but there have no studies determining whether this is the case in spatially structured populations. We use eDNA to structure bacterial cells and study QS within two distinct forms of aggregate formation: a laddered organized structure and a clumping disorganized structure. We find that QS-negative and QS-positive strains grow together in laddered structures, allowing us to design studies to quantify QS interactions at the single-cell level. We also have begun work with a QS-reporter to determine how structuring impacts the strength of QS."
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- Created By: mavdonina3
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- Created On: Oct 23, 2020 - 9:29am
- Last Updated: Oct 23, 2020 - 9:29am
