Biopolymer Characterization Core Seminar

Event Details
  • Date/Time:
    • Thursday February 20, 2020
      12:00 pm - 1:00 pm
  • Location: Krone Engineered Biosystems Building, Room 5029 - Atlanta, GA
  • Phone:
  • URL:
  • Email:
  • Fee(s):
    N/A
  • Extras:
Contact

Dalia Gulick and Bettina Bommarius - hosts

Summaries

Summary Sentence: Isothermal Titration Calorimeters & Differential Scanning Calorimeters

Full Summary: No summary paragraph submitted.

This presentation will cover the principles of operation and theory of the methods; experimental design and sample requirements; examples of applications in the study of binding interactions of biological molecules and their thermal stability.  Light refreshments served. RSVP by 02/13/2020 to ernie.fox@malvern.com 

Test for binding affinity with ITC or thermal stability with DSC 

  • No label needed 
  • Done directly in solution 
  • Information you get from ITC: KD , stoichiometry...
  • Information you get from DSC: Tm ...

Some of the sample types that can be tested on the MicroCal PEAQ ITC and DSC: 

ITC 

  • Protein, protein interactions 
  • Protein, small molecule interaction 
  • Protein, DNA and RNA interaction 
  • Nanoparticles, protein interaction 
  • Nanoparticles, DNA/ RNA interaction 
  • And more… 

DSC 

  • Protein 
  • Nucleic acids 
  • Liposomes 
  • Virus or VLP 
  • And more… 

Related Links

Additional Information

In Campus Calendar
Yes
Groups

Parker H. Petit Institute for Bioengineering and Bioscience (IBB)

Invited Audience
Faculty/Staff, Postdoc, Graduate students
Categories
Seminar/Lecture/Colloquium
Keywords
IBB, go-PetitInstitute
Status
  • Created By: Floyd Wood
  • Workflow Status: Draft
  • Created On: Feb 10, 2020 - 11:04am
  • Last Updated: Feb 11, 2020 - 1:21pm